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In Vivo Neuromethods

In Vivo Neuromethods
In Vivo Neuromethods By Alan A. Boulton, Glen B. Baker, Alan N. Bateson (auth.)
1998 | 396 Pages | ISBN: 0896035115 | PDF | 16 MB
In Vivo Neuromethods is the first of three successor works that update and expand the best-selling inaugural volume of Humana Press's warmly-received Neuromethods series, General Neurochemical Techniques (vol. 1). This important new book offers critical reviews of key techniques for investigating nervous system function, as well as the actions of drugs on the nervous system. The surveys range from authoritative treatment of such well-established techniques as the recording of electrical activity in the brain, to practical coverage of state-of-the-art methods, including neural transplantation and antisense technology. The techniques described are particularly suitable for studying the mechanisms of action of psychotropic drugs and drugs of abuse, as well as the etiology and pharmacotherapy of neuropsychiatric diseases and neurodegenerative disorders. Several of the methods detailed are highly useful in screening potential drugs for the treatment of depression, anxiety disorders, schizophrenia, and Parkinson's and Alzheimer's disease. The companion volumes, Molecular Neurobiology Techniques and Cell Neurobiology Techniques concentrate on in vitro approaches. Like their original predecessor, all three cutting-edge works will prove exceptionally useful to those basic and clinical neuroscientists who want to expand the range of their current research, or to develop competence in complimentary methods.

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In Vitro Neurochemical Techniques

In Vitro Neurochemical Techniques
In Vitro Neurochemical Techniques By Martin Davies, Susan M. j. Dunn (auth.), Alan A. Boulton, Glen B. Baker, Alan N. Bateson (eds.)
1999 | 296 Pages | ISBN: 0896035093 | PDF | 22 MB
In Vitro Neurochemical Techniques is the third work updating and expanding the best-selling inaugural volume of Humana Press's warmly received Neuromethods series, General Neurochemical Techniques (vol. 1). The key techniques detailed in this new edition encompass the breadth of neurochemical and molecular neurobiology research, ranging from the isolation of neuronal genes and the study of their expression to the analysis of receptor-ligand interactions, to the characterization of the consequences of receptor activation. The methods include electrophysiological techniques to explore the functional properties of receptors present in the membranes of excitable cells, methods to isolate novel genes central to neurobiological processes, and protocols to perform in situ hybridization histochemistry. Other methods cover the measurement of changes in gene expression, the rapid identification of gene polymorphisms, and the identification and characterization of second messenger pathways. The companion volumes, In Vivo Neuromethods and Cell Neurobiology Techniques, cover both in vivo methods and in vitro cell neurobiology approaches. Like the original, all three cutting-edge works will prove exceptionally useful to those basic and clinical neuroscientists who want to expand the range of their current research or develop competence in complementary methods.

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In Situ Hybridization Protocols

In Situ Hybridization Protocols
In Situ Hybridization Protocols By Nicoletta Archidiacono, Rachele Antonacci, Antonino Forabosco, Mariano Rocchi (auth.), K. H. Andy Choo (eds.)
1995 | 483 Pages | ISBN: 0896032809 | PDF | 18 MB
Thirty-one detailed, practical chapters many by the originators themselves present easy-to-follow protocols of in situ hybridization techniques for mapping DNA sequences onto chromosomes, quantitation/localization of RNA in tissues, and detection of virus nucleic acids. The methods serve molecular biologists in most areas of basic and applied research, and in every sort of organism ranging from viruses to Drosophila to humans. The expert contributors provide step-by-step instructions for the preparation of probes for DNA, teleomeric repeats, cDNA sequences, small synthetic oligomers, whole cosmids, mega-size YACs, and chromosome-specific paints from somatic cell hybrids and flow-sorted chromosomes. They also present radioactive and nonradioactive probe labeling procedures with the use of nick translation, random priming, multicolor labeling, PRINS labeling of DNA and RNA, and PCR in situ hybridization. Color illustrations enhance the text. Troubleshooting tips, alternative ways of doing things, and informative explanations of why certain steps are done -- aids not usually found in standard journal recipes -- guarantee a significant difference in the outcome of experiments.

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How to Be a Great Astrologer The Planetary Aspects Explained

How to Be a Great Astrologer The Planetary Aspects Explained
James Braha, "How to Be a Great Astrologer: The Planetary Aspects Explained"
English | ISBN: 0935895027 | | 256 pages | EPUB | 475 KB
New, from the author of ANCIENT HINDU ASTROLOGY FOR THE MODERN WESTERN ASTROLOGER, a hard hitting, plain spoken guide to interpreting the planetary aspects in Western astrology. Braha pulls no punches in this extremely clear, accessible reference text. It is the most comprehensive work on the subject to date, & includes 23 descriptive pages on the Moon s nodes in the signs & houses. Exquisitely designed, with gold embossed cover & 21 full size Gustave Dore illustrations. DIAMOND FIRE ASTROLOGY MAGAZINE SAYS: "This book is more than just another well written book on aspects. It is a work of art. The book is lavishly illustrated with angelic, celestial pictures. And it includes quotes & aphorisms from the great figures of this age. The whole effect is to put the reader in an exalted frame of mind - a frame of mind where real learning & inspiration take place. The art alone is worth the price of the book. The aspect delineations are well written & astrologically accurate. The section on the nodes is one of the clearest explanations I have ever seen in print!" Invaluable to professionals & amateurs alike.

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Hemostasis and Thrombosis Protocols

Hemostasis and Thrombosis Protocols
Hemostasis and Thrombosis Protocols By K. John Pasi (auth.), David J. Perry, K. J. Pasi (eds.)
1999 | 368 Pages | ISBN: 0896034194 | PDF | 3 MB
In Hemostasis and Thrombosis Protocols, leading experimentalists describe in detail their proven, cutting-edge methods for research on hemostasis and thrombosis, as well as their diagnostics. The techniques range from widely used basic assays to methods that are specialized for mutational analysis and specific disorders. Readily reproducible, these powerful methods can be used to screen for such inherited disorders as hemophilia A and B, von Willebrands Disease, Factor XI and antithrombin deficiency, protein S deficiency, factor V Leiden mutation, and other problems. The protocols reflect the many major advances that have occurred in our understanding of the molecular genetics of normal hemostasis and it variants, as well as of the coagulation cascade itself. Suitable for both diagnostic and research laboratories, Hemostasis and Thrombosis Protocols enables experienced and novice investigators alike readily to master the complexities of research on molecular hemostasis and thrombosis, as well as work toward the development of productive new molecular diagnostics.

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